Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania

Simple item page
dc.contributor.authorLukambagire, AS
dc.contributor.authorMendes, AJ
dc.contributor.authorBodenham, RF
dc.contributor.authorMcGiven, JA
dc.contributor.authorMkenda, NA
dc.contributor.authorMathew, C
dc.contributor.authorRubach, MP
dc.contributor.authorSakasaka, P
dc.contributor.authorShayo, DD
dc.contributor.authorMaro, VP
dc.contributor.authorShirima, GM
dc.contributor.authorThomas, KM
dc.contributor.authorKassanga, CJ
dc.contributor.authorKazwala, RR
dc.contributor.authorHalliday, JEB
dc.contributor.authorMmbaga, BT
dc.date.accessioned2021-05-11T11:54:26Z
dc.date.available2021-05-11T11:54:26Z
dc.date.issued2021
dc.description.abstractThe control of brucellosis across sub-Saharan Africa is hampered by the lack of standardized testing and the use of tests with poor performance. This study evaluated the performance and costs of serological assays for human brucellosis in a pastoralist community in northern Tanzania. Serum collected from 218 febrile hospital patients was used to evaluate the performance of seven index tests, selected based on international recommendation or current use. We evaluated the Rose Bengal test (RBT) using two protocols, four commercial agglutination tests and a competitive enzymelinked immunosorbent assay (cELISA). The sensitivity, specificity, positive predictive value, negative predictive value, Youden’s index, diagnostic accuracy, and per-sample cost of each index test were estimated. The diagnostic accuracy estimates ranged from 95.9 to 97.7% for the RBT, 55.0 to 72.0% for the commercial plate tests, and 89.4% for the cELISA. The per-sample cost range was $0.69–$0.79 for the RBT, $1.03–$1.14 for the commercial plate tests, and $2.51 for the cELISA. The widely used commercial plate tests performed poorly and cost more than the RBT. These findings provide evidence for the public health value of discontinuing the use of commercial agglutination tests for human brucellosis in Tanzania.en_US
dc.description.sponsorshipDELTAS Africa Initiative Afrique One-ASPIRE scholarship scheme (Afrique One-ASPIRE/DEL-15-008, http://afriq ueone aspir e.org). Â.J.M is supported by The University of Glasgow’s Lord Kelvin/Adam Smith (LKAS) PhD scholarship. R.F.B received scholarship support from the UK Biotechnology and Biological Sciences Research Council (BBSRC), Department for International Development (DFID), the Economic & Social Research Council, the Medical Research Council, the Natural Environment Research Council and the Defence Science & Technology Laboratory, under the Zoonoses and Emerging Livestock Systems – Associated Studentship (ZELS-AS) programme (grant number BB/N503563/1). This study was also supported by the Zoonoses and Emerging Livestock Systems program grant numbers BB/L018845 and BB/ L017679 http://www.bbsrc .ac.uk/).en_US
dc.identifier.other| https://doi.org/10.1038/s41598-021-82906-w
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/3506
dc.language.isoenen_US
dc.publisherNature scientific Reporten_US
dc.subjectserological testsen_US
dc.subjectbrucellosis controlen_US
dc.subjectpastoralist communityen_US
dc.subjectpublic healthen_US
dc.subjectTanzaniaen_US
dc.titlePerformance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzaniaen_US
dc.typeArticleen_US
dc.urlhttps://www.nature.com/articles/s41598-021-82906-wen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Abdulhamid.pdf
Size:
1.41 MB
Format:
Adobe Portable Document Format
Description:
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.67 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Articles, Conferences and Workshop Papers Collection