Browsing by Author "Bakari, G. G."

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Consumer knowledge, attitudes, and practices towards meat adulteration in Kilosa, Tanzania
(East African Journal of Science, Technology and Innovation, 2025-03) Dule, E. J.; Kinimi, E.; Mushi, J. R.; Max, R. A.; Bakari, G. G.; Lyimo, C. M.
Meat adulteration refers to the intentional or unintentional substitution, mislabeling, or contamination of meat products with lower-quality or undeclared animal species, additives, or non-meat substances. This practice can lead to serious consequences, including food safety risks, economic fraud, loss of consumer trust, and potential health hazards such as allergic reactions or exposure to harmful contaminants. The fight against meat adulteration in most developing countries is still hindered by a lack of consumer awareness of the concepts of meat adulteration and a clear description that constitutes a legal case of this fraudulent act. Limited studies on consumer awareness, perception and buying practices regarding meat adulteration in Tanzania made this study particularly interesting. From December 2023 to March 2024, a survey of 384 individuals in Kilosa District revealed that 95.1% of consumers had a low knowledge of meat adulteration (mean score: 4.8 ± 1.4). Multiple linear regression analysis revealed five significant predictors of consumers’ knowledge: age (31–45 years), education level (tertiary), occupation (butcher and business), employment status, and residency time (1–10 years). Notably, 65.4% were unaware of legislation addressing meat adulteration in Tanzania, and 87.7% linked the upsurge of adulteration practices to economic incentives. Additionally, consumer meat purchasing decisions were primarily influenced by price (86.2%) and appearance (52.9%) of the meat. This study highlights a critical lack of knowledge and practices of identifying adulterated meat in most of the consumers in Kilosa district. Therefore, the urgent need for targeted consumer education and stricter regulatory enforcement to combat meat adulteration in Tanzania is suggested.
Evaluation of stress hormone (cortisol) levels and some biochemical parameters of pigs kept under intensive management systems in Morogoro, Tanzania
(TANZANIA VETERINARY ASSOCIATION, 2017) Bakari, G. G.; Mollel, E.; Max, R. A.; Muhairwa, A. P.
The worldwide increase in demand for animal products in recent decades has necessitated raising of food animals under intensive systems that have been demonstrated to cause stress to animals. A cross sectional study was carried conducted to evaluate the welfare of pigs kept under intensive system using serum cortisol levels and some biochemical parameters as indicators of stress. A total of 302 pigs, aged three months to two years, from urban and peri-urban areas of Morogoro township were purposively involved in the study which assessed farm management aspects (housing and feeding) and blood levels of cortisol and some biochemical parameters (glucose, total protein and cholesterol). Results showed that serum cortisol levels were significantly higher (p < 0.001) in pigs from the peri-urban farms than those dwelling in the urban settings. Weaner pigs and boars had the highest serum cortisol levels compared to other groups. Significantly high levels of plasma cholesterol (p < 0.05) were measured in boars followed by gilts and weaners all kept in the urban areas whereas no significant differences were observed as far as plasma glucose and total proteins were concerned. Result on farm management assessment showed that urban piggery housing was of better quality than in the peri-urban and that feeding was largely influenced by local availability of the major feed ingredients such as maize bran and vegetable residues. About 80% of urban pigs were maintained on high concentrate feed due to availability of swills (restaurant leftovers) and maize bran whereas low concentrate feed with mainly vegetable residues dominated in the peri-urban settings. It is concluded that, serum cortisol levels observed in the current study were a reflection of stress to different groups of pigs kept under the intensive system and that feeds had some direct effect on biochemical parameters such as plasma cholesterol levels.
In-vitro wound healing properties of commiphora swynnertonii resinous extracts
(2021-09) Bakari, G. G.; Mshamu, S.A; Ally, M. H; Max, R.A; Bai, H
Wound healing is a complex multicellular process involving many cell types which include; inflammatory cells, endothelial cells, fibroblasts and keratinocytes. The process involves an orderly sequence of events with four overlapping phases namely; haemostasis, inflammatory, proliferation and remodeling phases. The process can be facilitated by the use of wound healing agents including herbal remedies from plants. In this study the main objective was to evaluate the in vitro wound healing activity of the resin obtained from Commiphora swynnertonii (C.swynnertonii). First the NIH -3T3 cells viability were evaluated using (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl Tetrazolium Bromide (MTT) assay. Then the wound scratch assay model was used to evaluate cellular proliferation, closure of the wound and release of matrix metalloproteinase enzymes. Results indicate differences in mean cell viability between different concentrations within 24 hours of incubation. The highest viability was recorded at the concentration of 1% (v/v). The in-vitro wound scratch assay showed positive NIH - 3T3 cells proliferation on the wound area and cells migration when compared with control group (without treatment) at 0 and 24 hours. In addition, C. swynnertonii was able to stimulate secretion of MMP-2 release from NIH - 3T3 cells. MMP-2 is an important enzyme for extracellular matrix remodeling during wound healing suggesting that C. swynnertonii promotes wound healing by stimulating cell proliferation and production of MMP-2 in a mechanism that is currently not known.