Browsing by Author "Chenyambuga, Sebastian"

Now showing 1 - 5 of 5

Abundance and distribution of ixodid tick species Infesting cattle reared under traditional farming Systems in Tanzania
(African Journal of Agricultural Research, 2017) Kerario, Isack Ibrahim; Muleya, Walter; Chenyambuga, Sebastian; Koski, Marja; Hwang, Seong-Gu; Simuunza, Martin
Ticks and tick-borne diseases are serious constraints to livestock production in Tanzania and other sub-Saharan African countries. Despite this, knowledge on the abundan ce of tick species infesting cattle in most parts of Tanzania is insufficient or lacking. This study was conducted to identify species and establish the abundance of ticks infesting cattle in Mara, Singida and Mbeya regions of Tanzania. The ticks were collected from one side of the body, counted and identified, based on morphological characteristics; to species level. The mean tick count per animal was significantly higher in Mara (35.8±4.3, p=0.0001) as compared to Singida (12.9±2.1) and Mbeya (7.0±0.4) regions. Young animals in Mara (24.7±6.0, p=0.0395) and Mbeya (5.4±0.3, p=0.0252) exhibited relatively lower mean tick counts compared to the weaners (Mara = 33.8±6.5, Mbeya = 7.2±0.7) and adult animals (Mara = 46.3±8.4, Mbeya = 7.8±0.7). Seven tick species from three different genera, namely Ambylomma, Hyalomma, Rhipicephalus (including the subgenus Boophilus), were identified. However, only five species (A. lepidum, A. variegatum, R. decoloratus, R. microplus and H. rufipes) were observed in all the three regions. R. appendiculatus and R. evertsi were not found in Mbeya and Mara respectively. The most prevalent species in Mara, Singida and Mbeya were R. appendiculatus (50.5%), A. lepidum (31.2%) and R. evertsi (35.6%), respectively. This study showed the existence of a variety of tick species, most of them being of veterinary importance. Therefore, strategic planning and cost-effective tick control measures should be implemented in order to reduce losses caused by ticks and tick borne diseases in the study area.
Comparative growth and survival performance of sea cucumber (Holothuria scabra) in co-cultured pen system with commercial macroalgae
(African Journal of Biological Sciences [Associate Organisation], 2019-10) Hamad, Muumin I.; Mwandya, Augustine W.; Munubi, Renalda S.; Chenyambuga, Sebastian; Lamtane, Heiromin A.
Mariculture has recently been adopted in many parts of coastal East Africa as a source of income and employment to many women and heartbroken fishermen who are the main victim of dwindling wild stock of aquatic resources. The sea cucumber (Holothuria scabra) has for long time been collected and sold as export marine product. Macroalgae (Eucheuma denticulatum and Kappaphycus alvarezii) are the common cultured seaweed species that provide hope for future increase in mariculture production. An experiment was conducted along the intertidal lagoon of Unguja Ukuu village in Zanzibar to assess the survival and growth performance of sea cucumber (H. scabra) in two separate pens under co-cultured systems with E. denticulatum and K. alvarezii. Juvenile sea cucumber H. scabra with mean weight (± se) of 67.18 ± 2.06 were integrated with the two common commercial seaweed in pen system for 10 weeks. The results revealed that the growth rate and survival of H. scabra, E. denticulatum and K. alvarezii were better under integration system. The growth of H. scabra was higher (1.038 g d-1) in pen systems co-cultured with K. alvarezii compared to 0.898 gd-1 in pen systems co-culture with E. denticulatum. Survival rate of H. scabra was higher (76%) in the pen systems co-cultured with E. denticulatum compared to that (70%) observed in pen systems co-cultured with K. alvarezii. The results suggest that the best integration of sea cucumber and macroalgae is between H. scabra and K. alvarezii. However, reliable source of H. scabra juvenile is essential for the future expansion of pen co-culture system.
Exploring knowledge and management practices on ticks and tick-borne Diseases among agro-pastoral communities in southern highlands, Tanzania
(2018) Kerario, Isack Ibrahim; Simuunza, Martin; Laisser, Emmanuel L. K.; Chenyambuga, Sebastian
Aim: The current study was conducted to assess the farmers’ knowledge and management practices on ticks and tick-borne diseases (TBDs) through individual interview using a structured questionnaire in Mbarali and Momba districts of Mbeya region. Materials and Methods: A total of 240 households, 120 from each district were asked to mention TBDs of cattle which they thought were the most important in their localities and period of the year when the diseases occurred more frequently. In addition, farmers were asked to describe clinical signs and management practices associated with the common TBDs that they knew. Results: The majority of respondents (46.2%) reported that East Coast fever (ECF) was the most important disease of cattle in the region, followed by anaplasmosis (33.8%), heartwater (15.4%), and babesiosis (4.6%). According to the farmers, ECF and anaplasmosis occurred more frequently during the dry season, while babesiosis and heartwater occurred more frequently during the rainy season. The majority of farmers were able to describe properly the signs of the common TBDs. Most farmers (80.4%) reported that they used acaricide to control ticks at a frequency of after every 2 weeks and a small proportion (15.8%) vaccinated their animals against ECF. Conclusion: It can be concluded that farmers in Mbeya have considerable knowledge on tick species and clinical signs of TBDs affecting their cattle. Based on the findings of the current study, it is recommended that integrated approach to the control of ticks and TBDs be adopted in the study area and many other areas that utilize agro-pastoral and pastoral cattle production systems.
Polymorphisms of BoLA-DRB 3.2 gene and associated genetic relationships among four strains of Tanzania shorthorn zebu cattle
(2018) Lubambe, Dominick; Msalya, George; Kipanyula, Maulilio; Karimuribo, Esron; Chenyambuga, Sebastian
Bovine Lymphocyte Antigen (BoLA) genes play important roles in resistance or susceptibility of cattle to infectious diseases. The BoLA gene comprises of several loci including the most polymorphic site namely DRB 3.2. We amplified 200 DNA samples and sequenced 270 bp comprising exon 2 of BoLA-DRB 3.2 in four strains of Tanzania shorthorn zebu (TSZ) cattle (Tarime, Sukuma, Maasai, Singida white) and one breed, namely Friesian. Sequences were processed on Finch TV, aligned on Basic Local Alignment Search Tool (BLAST) online, and matched to amino acids using MEGA 6. Frequency of each allele was computed as proportion of total alleles in each population. Chi-square was used to test significance in allele frequencies. Heterozygosities were computed using Poptree 2. Putative evolutionary relationships were evaluated by Nei genetic distances. Thirty four alleles were determined, of which nine alleles are novel. The greatest number of alleles was determined in Tarime and Singida white and the lowest in Friesian. Heterozygosities were high within the animals. Phylogenetic tree showed two major clusters one for TSZ and a second for Friesian. Polymorphism at DRB 3.2 in TSZ could be one explanation for their ability to withstand various diseases and we recommend further evaluations in the breed
Seasonal variations of nematode infection in Small East African goats and their crosses with Boer and Saanen reared under extensive and semi- intensive systems
(2010-04) Muhikambele, Vedasto; Chenyambuga, Sebastian; Mbaga, Said
A study was conducted in Turiani (sub-humid environment) and Mlali (semi-arid environment) to assess the seasonal variation of nematode infection in Small East African (SEA) goats and F 1 crosses of SAE with Saanen and Boer. The SEA goats were kept under extensive system while the crossbreds were kept under semi-intensive system. In Mlali 37 SEA goats and 30 SEA x Boer crosses while in Turiani 30 SEA goats and 33 SEA x Saanen crosses were included in the study. Worm burden was assessed using faecal egg count (FEC) and packed cell volume (PCV) as indicator traits. Faecal and blood samples were collected at the end of dry season, mid and end of rain season. The dominant worm species were identified by faecal culture. The results indicated that fewer animals (30 – 66.7%) were infected at the end of the dry season than at the mid and end of the rain season (69.7 – 100%). The FEC values differed significantly between periods of the year (P< 0.001) and between locations (P<0.05). The geometric mean faecal egg count (GFEC) ranged from 71.3 to 200.9, 185.8 to 516.4 and 273.5 to 924.7 eggs per gram (epg) at the end of dry season, mid and end of rain season, respectively. The GFEC values of SEA goats reared under extensive system were slightly higher (80.5 – 924.7 epg) than those of crossbred goats (71.3 – 690.2 epg) reared under semi-intensive system. The PCV values differed significantly (P<0.001) between locations, periods of the year and breeds. In Mlali, the PCV values ranged from 18 to 45% while in Turiani, the values ranged from 10 to 43%. The dominant parasite in the study areas was Haemonchus spp and accounted for 47.5 and 48.9% of total worms in Turiani and Mlali, respectively. This was followed by Trichostrongylus spp (21.3 and 19.8% in Mlali and Turiani, respectively) and Oesophagostomum spp (18.2 and 20.7% in Turiani and Mlali, respectively). The proportions of Strongyloides spp (6%), Bunostomum spp (4%) and Cooperia spp (3%) were small in all periods and locations. It is concluded that the level of nematode infection is highest at the end of the rain season and low during the dry season. The crossbred goats kept under semi-intensive grazing system had lower level of nematode infection than the local goats kept under the free-range grazing system