Browsing by Author "Karimuribo, Esron D"

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Analysis of host genetic factors influencing African trypanosome species infection in a cohort of Tanzanian Bos indicus cattle
(Elsevier, 2011) Karimuribo, Esron D; Morrison, Liam J.; Black, Alana; Turner, Michael R.; Kambarage, Dominic M.; Ballingall, Keith T.
Trypanosomosis caused by infection with protozoan parasites of the genus Trypanosoma is a major health constraint to cattle production in many African countries. One hundred and seventy one Bos indicus cattle from traditional pastoral Maasai (87) and more intensively managed Boran (84) animals in Tanzania were screened by PCR for the presence of African animal trypanosomes (Trypanosoma congolense, Trypanosoma vivax and Trypanosoma bru- cei), using blood samples archived on FTA cards. All cattle screened for trypanosomes were also genotyped at the highly polymorphic major histocompatibility complex (MHC) class II DRB3 locus to investigate possible associations between host MHC and trypanosome infec- tion. Overall, 23.4% of the 171 cattle tested positive for at least one of the three trypanosome species. The prevalence of individual trypanosome species was 8.8% (T. congolense), 4.7% (T. vivax) and 15.8% (T. brucei). The high prevalence of T. brucei compared with T. congolense and T. vivax was unexpected as this species has previously been considered to be of lesser impor- tance in terms of African bovine trypanosomosis. Significantly higher numbers of Maasai cattle were infected with T. brucei (23.0%, p = 0.009) and T. congolense (13.8%, p = 0.019) com- pared with Boran cattle (8.3% and 3.6%, respectively). Analysis of BoLA-DRB3 diversity in this cohort identified extensive allelic diversity. Thirty-three BoLA-DRB3 PCR-RFLP defined alle- les were identified. One allele (DRB3*15) was significantly associated with an increased risk (odds ratio, OR = 2.71, p = 0.034) of T. brucei infection and three alleles (DRB3*35, *16 and *23) were associated with increased risk of T. congolense infection. While further work is required to dissect the role of these alleles in susceptibility to T. brucei and T. congolense infections, this study demonstrates the utility of FTA archived blood samples in combined molecular analyses of both host and pathogen.
Are we prepared for emerging and re-emerging diseases? Experience and lessons from epidemics that occurred in Tanzania during the last five decades
(2011-12) Rweyemamu, Mark M; Mmbuji, Peter; Kivaria, Fredrick M; Simba, Azma; Mbugi, Erasto; Karimuribo, Esron D; Mboera, Leonard E.G
This paper reviews preparedness for containing and controlling emerging and re-emerging diseases drawing lessons from disease events that occurred in animal and human populations in the last five decades (1961-2011). A comprehensive analysis based on retrieval and analysis of grey and published literature as well as reported cases was carried out to document type and trend of occurrence of emerging and re-emerging infectious diseases in different parts of Tanzania. Overall, the majority of diseases reported in the country were viral in nature followed by bacterial diseases. The trend for the occurrence shows a number of new emerging diseases as well as re-occurrence of old diseases in both animal (domestic and wild) and human populations. In humans, the major disease epidemics reported in the last five decades include cholera, influenza A H1N1, plague and rubella. In animals, the major epidemic diseases reported were Contagious Bovine Pleuropneumonia, Contagious Caprine Pleuropneumonia, Peste des petits ruminants and Giraffe Ear and Skin Diseases. Some epidemics have been reported in both human and animal populations including Rift Valley fever and anthrax. The emergence of the ‘fit-for purpose’ approaches and technologies such as the discipline of One Health, use of participatory epidemiology and disease surveillance and mobile technologies offers opportunity for optimal use of limited resources to improve early detection, diagnosis and response to disease events and consequently reduced impact of such diseases in animal and human populations.
Determination of genetic structure and Signatures of selection in three strains of Tanzania shorthorn zebu, boran and friesian Cattle by genome-wide snp analyses
(PLOS, 2017) Msalya, George; Kim, Eui-Soo; Laisser, Emmanuel L. K; Kipanyula, Maulilio J; Karimuribo, Esron D; Kusiluka, Lughano J. M; Chenyambuga, Sebastian W; Rothschild, F
More than 90 percent of cattle in Tanzania belong to the indigenous Tanzania Short Horn Zebu (TSZ) population which has been classified into 12 strains based on historical evi- dence, morphological characteristics, and geographic distribution. However, specific genetic information of each TSZ population has been lacking and has caused difficulties in designing programs such as selection, crossbreeding, breed improvement or conservation. This study was designed to evaluate the genetic structure, assess genetic relationships, and to identify signatures of selection among cattle of Tanzania with the main goal of under- standing genetic relationship, variation and uniqueness among them.
Epidemiological investigation into the introduction and factors for spread of Peste des Petits Ruminants, southern Tanzania:
(AOSIS, 2012-01) Muse, Epaphras A; Karimuribo, Esron D; Misinzo, Gerald; Mellau, Lesakit SB; Msoffe, Peter LM; Gitao, George C; Swai, Emmanuel S
A study was carried out to confirm and identify sources and elucidate factors associated with the introduction of Peste des Petits Ruminants (PPR) in southern Tanzania. This study was conducted in Tandahimba and Newala districts of Mtwara region following suspected outbreak of PPR in the area. Qualitative data were collected using semi-structured questionnaires and in-depth interviews of key informants who included goat and sheep owners with suspected cases of PPR and animal health service providers as well as local administrative authority. Additionally, 216 serum samples and 28 swabs were collected for serological and virological laboratory disease confirmation. The results show that PPR was first introduced in Likuna village of Newala district in February 2009 through newly purchased goats from the Pugu livestock market located about 700 km in the outskirts of Dar es Salaam city …
Exploring local knowledge and perceptions on zoonoses among pastoralists in northern and eastern Tanzania
(PLOS, 2017-02) Mangesho, Peter Ernest; Neselle, Moses Ole; Karimuribo, Esron D; Mlangwa, James E; Queenan, Kevin; Mboera, Leonard E. G; Rushton, Jonathan; Kock, Richard; Häsler, Barbara; Kiwara, Angwara; Rweyemamu, Mark
Zoonoses account for the most commonly reported emerging and re-emerging infectious diseases in Sub-Saharan Africa. However, there is limited knowledge on how pastoral com- munities perceive zoonoses in relation to their livelihoods, culture and their wider ecology. This study was carried out to explore local knowledge and perceptions on zoonoses among pastoralists in Tanzania.
Knowledge, attitude and practices of frontline health workers in relation to detection of brucellosis in rural settings of Tanzania: a cross-sectional study
(BMC, 2022) Belinda, Joseph Mligo; Calvin, Sindato; Yapi, Richard B; Mathew, Coletha; Mkupasi, Ernatus; Kazwala, Rudovick R; Karimuribo, Esron D
Background: Brucellosis an important zoonotic disease worldwide, which frequently presents as an undiferentiated febrile illness with otherwise varied and non-specifc clinical manifestations. Despite its importance, there are few reports on its awareness among frontline health workers. This study aimed at assessing the baseline knowledge, atti tude and practice (KAP) related to detection and management of brucellosis among frontline health workers (FHWs) namely; healthcare workers (HWs) and community health workers (CHWs). Methods: A cross-sectional study was conducted from December 2019 to January 2020 in Kilosa and Chalinze dis tricts of Tanzania. Data on demographic characteristics, knowledge, attitude and practices regarding brucellosis were collected from the study participants using a structured questionnaire. Interviews were conducted with 32 HWs and 32 CHWs who were systematically selected in study districts. Chi square/fsher Exact was used to assess the associa tion between sociodemographic variables and those related to knowledge, attitude and practices. Results: Overall, a total of 30 (93.8%) HWs and nine (28.1%) CHWs from the study districts heard about brucello sis, with (34.4%) of HWs having knowledge about the causative organism. Overall, knowledge showed almost half (46.9%) HWs and (28.1%) CHWs were aware of the symptoms, clinical signs, diagnosis and control regarding brucel losis. Knowledge diference was statistically signifcant with HWs’ age (p=0.016). Almost half (46.9%) HWs and less than quarter (12.5%) CHWs had good practices regarding brucellosis control. Almost three quarters (71.9%) of HWs and (21.9%) CHWs had positive attitude regarding brucellosis control; overall attitude was statistically signifcant with CHWs age (p=0.028) and education level (p=0.024). Lack of awareness and unavailability of diagnostic tools were the main challenges faced by FHWs in the two districts. Conclusion: The majority of participants were not aware of human brucellosis. Moreover, their overall knowledge was inadequate and the common practices were diagnostic tools, and adequate knowledge to manage brucellosis cases. These fndings highlight the need to strengthen frontline health workers knowledge, practices and diagnostic capacities related to brucellosis