Browsing by Author "Kusiluka, Lughano Jeremy Moses"

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    Animal health constraints in dairy goats kept under Smallholder farming systems in Kongwa and Mvomero Districts, Tanzania
    (Journal of Veterinary Medicine and Animal Health, 2014) Shija, Dismas Said Ngasa; Kusiluka, Lughano Jeremy Moses; Chenyambuga, Sebastian Wilson; Shayo, Deogratias; Lekule, Faustin Paul
    This study was conducted to determine animal health constraints for dairy goats kept by small-scale farmers in Kongwa and Mvomero districts, Tanzania. A total of 129 dairy goats belonging to 108 farmers were screened for gastrointestinal nematode (GIN) infection, coccidiosis, haemoparasites, brucellosis and contagious caprine pleuropneumonia (CCPP) over a period of 11 months. Other clinical diseases and mortalities were recorded. The goats used were Norwegian crosses and Toggenburg crosses. The mean prevalence of GIN infection and coccidiosis in all goats were 54.8 and 57.4%, respectively. Prevalence of GIN infection was higher (P ≤ 0.05) during the rainy months than in the dry months, but the prevalence of coccidiosis did not differ (P > 0.05) between the dry and rainy seasons. The EPG in goats did not differ (P > 0.05) between Kongwa (169.79 ± 0.03 EPG) and Mvomero (171.51 ± 0.04 EPG) districts, but the OPG differed significantly (P ≤ 0.05) with values of 793.15 ± 0.04 (Kongwa) and 364.02 ± 0.05 (Mvomero). The prevalence of CCPP in the goats was 26.4%. Other clinical diseases included respiratory diseases, infectious keratoconjunctivitis and orf (scabby lesions around mouth and nostrils). Both tests for haemoparasites and brucellosis indicated negative results for all goats tested. Mortality rate during the study period was 15.5% and the major causes of deaths were respiratory diseases, bloat and food poisoning. In conclusion, gastrointestinal nematodes are prevalent in both districts, but the burdens are relatively low to justify mass treatment. The Norwegian goats are more susceptible to GIN infection and coccidiosis compared to Toggenburg goats.
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    In-vitro assessment of antibacterial effects of combined crude extracts of s. glaucescens and c. swynnertonii with antibiotics
    (Academic Journals, 2023) Ochollah, Mary George; Mabiki, Faith Philemon; Kusiluka, Lughano Jeremy Moses; Mdegela, Robinson Hammerthon; Olsen, John Elmerdahl
    Currently, there is an upsurge of bacterial resistance in single-drug treatment regimens. This has stimulated a growing interest in research and development of new antibacterial agents containing several ingredients as one of the means to combat bacterial resistance. Herb-antibiotic combination therapy is one of the reported effective treatment regimens to combat antimicrobial resistance. This study was aimed to assess antibacterial effects of combined crude extracts of Synadenium glaucescens and Commiphora swynnertonii with antibiotics. In this study, three standard antibiotic drugs namely, ciprofloxacin, ampicillin and erythromycin in combination with crude extracts from S. glaucescens and C. swynnertonii were screened for antibacterial effects against two Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis and three Gram-negative bacteria Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. Broth microdilution technique was used to determine the Minimum Inhibitory Concentration (MIC) while Fractional Inhibitory Concentration (FIC) indices were calculated from MIC values of combined extracts to determine the combination effects. Synergism was observed when ciprofloxacin was combined with all tested crude extracts against E. coli (ΣFIC of 0.02), combination of ciprofloxacin with extract from root barks of C. swynnertonii (ΣFIC of 0.5) against S. aureus, root barks of Synadenium glaucescens (ΣFIC of 0.1) against S. aureus and combination of ampicillin with all tested crude extracts (ΣFIC of 0.03-0.1) against E. faecalis. Moreover, antagonism was observed between the combinations of ampicillin and erythromycin with all tested crude extracts against Gram-negative bacteria (ΣFIC of 4-8). Therefore, the combinations which demonstrated synergism may be promising alternatives for the treatment of infectious diseases caused by E. coli, S. aureus and E. faecalis. However, in the future, toxicity studies for combinations which demonstrated synergism are recommended.