Browsing by Author "Lefebvre, D. J."

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomallysosomal system acidification
    (2007-12-17) Misinzo, G.; Delputte, P. L.; Lefebvre, D. J.; Nauwynck, H. J.
    Treatment of porcine kidney (PK-15) cells with either interferon-gamma (IFN-g) or endosomallysosomal system acidification inhibitors increases replication of porcine circovirus type 2 (PCV2). In the present study, the effect of a combination of these treatments on the number of infected cells and virus yield was tested. The number of PCV2 (Stoon-1010)-infected PK-15 cells increased in cells treated with ammonium chloride (445 39% increase), IFN-g (446 8%), ammonium chlorideþ IFN-g (1721 283%), chloroquine diphosphate (1037 121%), chloroquine diphosphateþIFN-g (2199 255%), monensin (950 178%) and monensinþIFN-g (1948 60%). Combined IFNg and endosomal-lysosomal system acidification inhibitors increased PCV2 yield by up to 50 times compared to untreated PK-15. This augmented virus replication in PK-15 cells may be helpful in the production of PCV2 vaccines.