Browsing by Author "Massawe, Deogracious Protas"

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Identification of potential seed storage protein responsible for bruchid resistance in common bean landraces from Tanzania and Malawi
(African Journal of Biotechnology, 2022-01) Maro, Caroline Ng’homange; Massawe, Deogracious Protas; Tryphone ,George Muhamba; Myers, James Robert; Davis, Joel William; Kusolwa, Paul Mbogo
Bean bruchids are among the most devastating insect pests of common bean that can inflict huge losses in storage. To identify potential resistance to these pests, screening was performed at Sokoine University of Agriculture. Two resistant landraces were identified, viz Kalubungula and KK25. Recombinant inbred (RI) KSy, KSw and ML populations were created from crosses between Soya × Kalubungula, Soworo × Kalubungula and Nagaga × KK25, respectively. Seed storage proteins were characterized and sequenced in RI population progenies to determine if phenotypic resistance was associated with α-amylase inhibitor – phytohemagglutinin – arcelin (APA) storage proteins. We found no association between the seed storage proteins observed in Kalubungula and its recombinant inbred lines with an APA protein. KK25 and its progenies had Arcelin-5, Leucoagglutinin, Erythroagglutinin and a hypothetical seed storage protein that conditions antibiosis effects as a resistance mechanism. The hypothetical seed storage protein observed in these lines may contribute to enhanced resistance.