Browsing by Author "Mathew, Coletha"

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Brucellosis testing patterns at health facilities in Arusha region, northern Tanzania
(PLOS ONE, 2022) Lukambagire, AbdulHamid Settenda; Shirima, Gabriel Mkulima; Shayo, Damas Davis; Mathew, Coletha; Yapi, Richard B.; Kasanga, Christopher Julius; Mmbaga, Blandina Theophile; Kazwala, Rudovick Reuben; Halliday, Jo E. B.
Background Brucellosis is listed as one of six priority zoonoses in Tanzania’s One Health strategic plan which highlights gaps in data needed for the surveillance and estimation of human brucello- sis burdens. This study collected data on current testing practices and test results for human brucellosis in Arusha region, northern Tanzania. Methods Retrospective data were extracted from records at 24 health facilities in Arusha region for the period January 2012 to May 2018. Data were captured on: the test reagents used for brucellosis, procurement and testing protocols, the monthly number of patients tested for brucellosis and the monthly number testing positive. Generalised linear mixed models were used to evaluate relationships between health facility characteristics and the probability that brucellosis testing was conducted in a given month, and the proportion of individuals testing positive. Results Four febrile Brucella agglutination tests were used widely. The probability of testing for bru- cellosis in a given month was significantly associated with an interaction between year of testing and facility ownership. Test probability increased over time with more pronounced increases in privately owned as compared to government facilities. The proportion of individ- uals testing positive for brucellosis was significantly associated with facility type and district, with individuals tested in hospitals in Meru, Monduli and Ngorongoro districts more likely to test positive. Conclusions Febrile Brucella agglutination tests, known for their poor performance, were the mainstay of brucellosis testing at health facilities in northern Tanzania. The study indicates that historical data on human brucellosis in Arusha and other regions are likely to provide an inaccurate measure of true disease burden due to poor performance of the tests used and variation in testing practices. Measures to address these identified shortcomings could greatly improve quality of testing and surveillance data on brucellosis and ultimately inform prevention and control of this priority disease.
Crossing the line: seroprevalence and risk factors for transboundary animal diseases along the Tanzania-Zambia border
(Veterinary Epidemiology and Economics, a section of the journal Frontiers in Veterinary Science, 2022) Lysholm, Sara; Lindahl, Johanna F.; Munyeme, Musso; Misinzo, Gerald; Mathew, Coletha; Alvåsen, Karin; Dautu, George; Linde, Siri; Mitternacht, Lydia; Olovsson, Emelie; Wilén, Elsa; Berg, Mikael; Wensman, Jonas J.
Transboundary pathogens pose a threat to livelihood security in countries such as Zambia and Tanzania. This study aimed to investigate the seroprevalence of peste des petits ruminants virus (PPRV), foot and mouth disease virus (FMDV), sheep and goat pox virus (SGPV), Rift Valley fever virus (RVFV) and Brucella spp. in sheep and goats along the Tanzania-Zambia border. Another aim was to assess the association between certain predictor variables and seroprevalence, focusing on trade and proximity to an international border, to a town and to the Tanzania-Zambia highway. During September-October 2018, 486 serum samples from small ruminants in Zambia and 491 in Tanzania were collected and analyzed using enzyme-linked immunosorbent assays (ELISA). A questionnaire focused on management strategies was administered to each household. The animal-level seroprevalence in Zambia was 0.21% [95% confidence interval (CI) (0.01–1.14) for PPRV, 1.03% (95% CI 0.33–2.39) for FMDV, 0% (95% CI 0–0.76) for SGPV, 2.26% (95% CI 1.14–4.01) for RVFV and 1.65% (95% CI 0.71–3.22) for Brucella spp.]. In Tanzania, animal-level seroprevalence was 2.85% (95% CI 1.57–4.74) for PPRV, 16.9% (95% CI 13.7–20.5) for FMDV, 0.20% (95% CI 0.01–1.13) for SGPV, 3.26% (95% CI 1.87–5.24) for RVFV and 20.0% (95% CI 14.5–26.5) for Brucella spp. For PPRV (OR 6.83, 95% CI 1.37–34.0, p = 0.019) and FMDV (OR 5.68, 95% CI 1.58–20.3, p = 0.008), herds situated more than 30 km from an international border were more likely to be seropositive, while being located 10–30 km (OR 4.43, 95% CI 1.22–16.1 p = 0.024) from a border was identified as a risk factor for Brucella spp. For FMDV (OR 79.2, 95% CI 4.52–1388.9, p = 0.003), being situated within 30 km from a town was associated with seropositivity. Furthermore, contact with wild ruminants (OR 18.2, 95% CI 1.36–244), and the presence of sheep in the household (OR 5.20, 95% CI 1.00–26.9, p = 0.049), was associated with seropositivity for PPRV, and FMDV. No significant associations between trade or distance to the Tan-Zam highway and seroprevalence were found. We recommend that the impact of trade and proximity to borders, towns and roads should be further evaluated in larger studies, ideally incorporating aspects such as temporal trade fluctuations.
Direct detection of brucella species in blood clots from live- stock in Northern Tanzania
(Sokoine University of Agriculture, 2021) AbdulHamid, S. Lukambagire; Akoko, James M.; Mathew, Coletha; Sambu, Rosamystica M.; Mwampashi, Raphael R.; Yapi, Richard; Amani, Nelson B.; Njau, Judith S.; Bonfoh, Bassirou; Shirima, Gabriel M.; Mmbaga, Blandina T.; Kasanga, Christopher J.; Katani, Robab; Halliday, Jo E.B.; Kazwala, Rudovick R.
Brucellosis is an endemic zoonotic disease of public health priority in many sub-Saharan countries, where robust tools for detection of Brucella in animal populations are needed for sur- veillance. Blood collected from 501 animals at a ranch suspecting brucellosis was tested for Brucella infection using molecular and serological techniques. Information on animal species, sex and abortion history were recorded. Blood clot DNA extracts were tested using two Brucella spp. genus specific targets, IS711 and bcsp31. Samples positive for both targets were subjected to a multiplex species-specific assay targeting alkB for B. abortus and BMEI1162 for B. melitensis. All sera were tested using the Rose Bengal test. Brucella spp. DNA was detected by qPCR in a total of 58 (11.6%) of 501 blood samples. B. abortus was identified in 18 and B. melitensis in 22 of the 58 samples posi- tive for Brucella spp. A total of 73 (14.6%) of 501 sera tested positive by RBT with poor agreement (kappa = 0.102) between the RBT and Brucella spp. qPCR assay results. Brucella abortus was found in cattle and goats, while B. melitensis was detected in cattle, sheep and goats. These findings sup- port the use of molecular assays alongside serology in brucellosis surveillance programs.
Gendered asymmetry of access to knowledge for brucellosis control among pastoral communities in north-west Côte d’Ivoire
(Pastoralism: Research, Policy and Practice, 2022) Babo, Stephane A. Y.; Fokou, Gilbert; Yapi, Richard B.; Mathew, Coletha; Dayoro, Arnaud K.; Kazwala, Rudovick R.; Bonfoh, Bassirou
Brucellosis is an infectious zoonotic disease considered as a threat to public health and pastoralist livelihoods. Symptoms of the disease can lead to gender-specific ailments such as abortions in women and orchitis in men. Pastoralists and their families are at high risk of contracting the disease. Access to health information reinforces existing knowledge and contributes to disease prevention. However, in developing countries, interventions for knowledge sharing on zoonotic diseases predominantly target men. This study aimed to describe mechanisms of knowledge production and transfer on brucellosis according to gender, by assessing the way knowledge affects behaviours of pastoral communities. A community-based cross-sectional survey was conducted among a pastoral community (PC) of the Folon region in north-west Côte d’Ivoire. The study included transhumant pastoralists, sedentary livestock owners, shepherds and their wives. By using mixed methods, 26 semi-structured interviews were conducted, and 320 questionnaires were completed. Statistical analysis with chi-square (χ2) comparison tests was performed to compare variables between men and women. Findings were interpreted through the concept of specialisation of the social exclusion theory. We found that gender influences access to information on brucellosis and transfer of knowledge on brucellosis appeared gender-biased, especially from veterinarians towards men in the community. The social labour division and interventions of veterinarians through awareness reinforce the knowledge gap on brucellosis between men and women. Men and women consume raw milk, whilst only men in general handle animal discharges with bare hands. To improve the control of brucellosis, knowledge on best practice should be shared with pastoral communities using the One Health approach that encourages mutual learning. Innovative strategies based on gender daily tasks such as safe dairy processing by women and safe animal husbandry to expand their herd for men can be the entry point for the prevention of brucellosis.
Knowledge Gain and Adoption among Farm Women through Interactive Multimedia in Kilosa and Sengerema Districts, Tanzania
(2021) Ngowi, Edwin; Mwakalobo, Adam; Mathew, Coletha
This paper examines knowledge gain and adoption among farm women with access to information and improved communication. This is because access to information and improved communication is a crucial requirement for agricultural development. In rural areas, group approach, “self-help groups (SHGs),” have been initiated among farm women using Interactive Multimedia Compact Disc (IMCD). The IMCD emerged as a potential learning tool to train farmers about a particular agricultural-knowledge. A theoretical and conceptual framework for an adoption pathway is reviewed in which farmers move from knowledge gain to adoption over time. This study was conducted in Sengerema and Kilosa districts with telecentres that train farm women in SHGs using IMCD. A total of 180 farm women were randomly and equally selected. Simple correlation and multiple regressions were used to assess association and influence of socioeconomic characteristics on knowledge gain and adoption. It was established that education status and mass media exposure exhibited a positive and significant relationship. Age and farm statuses showed negative association with knowledge gain. Farm status and innovativeness showed significant and positive relationship with knowledge adoption. Farming experience exhibited negative and significant relationship with knowledge adoption. Thus, while preparing IMCD, one should take care these variables for knowledge gain and adoption. The inferences on knowledge gain and adoption imply that longer periods will be required for majority of farm women to use the technology and to have the decision leading to adoption
Knowledge gain and adoption among farm women through interactive multimedia in Kilosa and Sengerema districts, Tanzania
(Tanzania Journal of Development Studies, 2021) Ngowi, Edwin; Mathew, Coletha; Mwakalobo, Adam
This paper examines knowledge gain and adoption among farm women with access to information and improved communication. This is because access to information and improved communication is a crucial requirement for agricultural development. In rural areas, group approach, “self-help groups (SHGs),” have been initiated among farm women using Interactive Multimedia Compact Disc (IMCD). The IMCD emerged as a potential learning tool to train farmers about a particular agricultural-knowledge. A theoretical and conceptual framework for an adoption pathway is reviewed in which farmers move from knowledge gain to adoption over time. This study was conducted in Sengerema and Kilosa districts with telecentres that train farm women in SHGs using IMCD. A total of 180 farm women were randomly and equally selected. Simple correlation and multiple regressions were used to assess association and influence of socioeconomic characteristics on knowledge gain and adoption. It was established that education status and mass media exposure exhibited a positive and significant relationship. Age and farm statuses showed negative association with knowledge gain. Farm status and innovativeness showed significant and positive relationship with knowledge adoption. Farming experience exhibited negative and significant relationship with knowledge adoption. Thus, while preparing IMCD, one should take care these variables for knowledge gain and adoption. The inferences on knowledge gain and adoption imply that longer periods will be required for majority of farm women to use the technology and to have the decision leading to adoption.
Knowledge, attitude and practices of frontline health workers in relation to detection of brucellosis in rural settings of Tanzania: a cross-sectional study
(BMC, 2022) Belinda, Joseph Mligo; Calvin, Sindato; Yapi, Richard B; Mathew, Coletha; Mkupasi, Ernatus; Kazwala, Rudovick R; Karimuribo, Esron D
Background: Brucellosis an important zoonotic disease worldwide, which frequently presents as an undiferentiated febrile illness with otherwise varied and non-specifc clinical manifestations. Despite its importance, there are few reports on its awareness among frontline health workers. This study aimed at assessing the baseline knowledge, atti tude and practice (KAP) related to detection and management of brucellosis among frontline health workers (FHWs) namely; healthcare workers (HWs) and community health workers (CHWs). Methods: A cross-sectional study was conducted from December 2019 to January 2020 in Kilosa and Chalinze dis tricts of Tanzania. Data on demographic characteristics, knowledge, attitude and practices regarding brucellosis were collected from the study participants using a structured questionnaire. Interviews were conducted with 32 HWs and 32 CHWs who were systematically selected in study districts. Chi square/fsher Exact was used to assess the associa tion between sociodemographic variables and those related to knowledge, attitude and practices. Results: Overall, a total of 30 (93.8%) HWs and nine (28.1%) CHWs from the study districts heard about brucello sis, with (34.4%) of HWs having knowledge about the causative organism. Overall, knowledge showed almost half (46.9%) HWs and (28.1%) CHWs were aware of the symptoms, clinical signs, diagnosis and control regarding brucel losis. Knowledge diference was statistically signifcant with HWs’ age (p=0.016). Almost half (46.9%) HWs and less than quarter (12.5%) CHWs had good practices regarding brucellosis control. Almost three quarters (71.9%) of HWs and (21.9%) CHWs had positive attitude regarding brucellosis control; overall attitude was statistically signifcant with CHWs age (p=0.028) and education level (p=0.024). Lack of awareness and unavailability of diagnostic tools were the main challenges faced by FHWs in the two districts. Conclusion: The majority of participants were not aware of human brucellosis. Moreover, their overall knowledge was inadequate and the common practices were diagnostic tools, and adequate knowledge to manage brucellosis cases. These fndings highlight the need to strengthen frontline health workers knowledge, practices and diagnostic capacities related to brucellosis
Prevalence of Brucella infection and associated risk factors among children in livestock keeping communities of Morogoro, Tanzania
(East African Journal of Science, Technology and Innovation, 2022) Hildegalda, C.; Komba, E.; Kazwala, R.; Mathew, Coletha
Brucellosis is among the most common bacterial zoonotic diseases worldwide. It is estimated that up to 500,000 new human cases occur each year. Brucellosis is a priority zoonosis in most East African countries. In Tanzania, the disease is one of six priority zoonoses, however, little disease burden information is available especially in children. Children are of particular interest especially in pastoral communities due to increased risk of exposure in their routine activities associated with handling of livestock and their products. This study investigated brucellosis exposure status and associated risk factors among non-febrile children in pastoral communities. A cross-sectional design was used involving a total of 361 non-febrile children of six months to 14 years old, randomly selected from households and schools. Risk practices were evaluated using focus group discussions among selected children and thematic analysis was performed. Serum samples were collected and analyzed using Rose Bengal Test (RBT) and indirect Enzyme-Linked Immunosorbent Assay (iELISA) in parallel. The overall seroprevalence of Brucella infection was 3.3% (95%CI, 2%-5%) determined by both RBT and iELISA. Risk practices observed and cited by children comprised of direct and indirect contact with animals and their products. These include: consumption of raw milk and their products, unhygienic handling of animals and their waste especially assisting cows during parturition. Girls/female respondents were more likely to test brucella positive than boys (male) respondents (2.23 OR, 95%CI 66%-754%). Brucellosis among non-febrile children in the study area was present at a relatively low prevalence. Risk practices for transmission of this zoonosis were also identified which included consumption of raw milk, contact with animals, their products and discharges including abortion materials which represent the basis for increased pathogen circulation in the area. General education on the epidemiology of brucellosis and prevention of infection spread should be provided to the community including children.