Browsing by Author "Olsen, J. E."

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Antimicrobial resistance in faecal samples from buffalo, wildebeest and zebra grazing together with and without cattle in Tanzania
(Journal of Applied Microbiology, 2014-11-28) Katakweba, A. A. S.; Møller, K. S.; Muumba, J.; Muhairwa, A. P.; Damborg, P.; Rosenkrantz, J. T.; Minga, U. M.; Mtambo, M. M. A.; Olsen, J. E.
Aim: The aim of this study was to determine whether the practice of co-grazing with cattle and wild life constitutes a risk of transmission of antibiotic resistant bacteria to wild ungulates. Methods and Results: Faecal samples were collected from buffalo (n = 35), wildebeest (n = 40), zebra (n = 40) and cattle (N = 20) from Mikumi National Park, Tanzania (MNP), where cattle is prohibited and from Ngorongoro Conservation Area (NCA) where co-grazing is practiced. The number of coliforms and enterococci resistant to selected antibiotics was determined. Wild life generally harboured higher number of resistant Escherichia coli and Enterococci than cattle, but with no general inﬂuence in wild life of co-grazing with cattle. Vancomycin-resistant Enterococci were detected in wild life samples, and E. coli resistant to cefotaxime and enroﬂoxacin were observed among isolates from all wild life, but not from cattle. Culture independent estimates of the number of sulII gene copies obtained by qPCR did not differ between wild life from the two sample sites, while tetW was signiﬁcantly higher in samples from MPN than from NCA. Conclusions: Antibiotic resistant bacteria were not more frequently found in ungulates grazing together with cattle than ungulates without this interaction. Signiﬁcance and Impact of the Study: This study did not indicate that transmission of antibiotic resistant bacteria is a frequent event following co-grazing of wild life and cattle.
Characterisation of commensal escherichia coli isolated from apparently healthy cattle and their attendants in Tanzania
(PLOS ONE, 2016-12-15) Madoshi, B. P.; Kudirkiene, E; Mtambo, M. M. A.; Muhairwa, A. P.; Lupindu, A. M.; Olsen, J. E.
While pathogenic types of Escherichia coli are well characterized, relatively little is known about the commensal E. coli flora. In the current study, antimicrobial resistance in commensal E. coli and distribution of ERIC-PCR genotypes among isolates of such bacteria from cattle and cattle attendants on cattle farms in Tanzania were investigated. Seventeen E. coli genomes representing different ERIC-PCR types of commensal E. coli were sequenced in order to determine their possible importance as a reservoir for both antimicrobial resistance genes and virulence factors. Both human and cattle isolates were highly resistant to tetracycline (40.8% and 33.1%), sulphamethazole-trimethoprim (49.0% and 8.8%) and ampicillin (44.9% and 21.3%). However, higher proportion of resistant E. coli and higher frequency of resistance to more than two antimicrobials was found in isolates from cattle attendants than isolates from cattle. Sixteen out of 66 ERIC-PCR genotypes were shared between the two hosts, and among these ones, seven types contained isolates from cattle and cattle attendants from the same farm, suggesting transfer of strains between hosts. Genome-wide analysis showed that the majority of the sequenced cattle isolates were assigned to phylogroups B1, while human isolates represented phylogroups A, C, D and E. In general, in silico resistome and virulence factor identification did not reveal differences between hosts or phylogroups, except for lpfA and iss found to be cattle and B1 phylogroup specific. The most frequent plasmids replicon genes found in strains from both hosts were of IncF type, which are commonly associated with carriage of antimicrobial and virulence genes. Commensal E. coli from cattle and attendants were found to share same genotypes and to carry antimicrobial resistance and virulence genes associated with both intra and extraintestinal E. coli pathotypes.
Current manure management practices and hygiene aspects of urban and peri-urban livestock farming in Tanzania
(Livestock Research for Rural Development,, 2012) Lupindu, A. M.; Ngowi, H. A.; Dalsgaard, A.; Olsen, J. E.; Msoffe, P. L. M.
The recent expansion of urban and peri urban livestock farming has resulted in close contact between animals and humans, sometimes with adverse human health effects. A survey involving 119 cattle keeping households in urban and peri-urban settings of Morogoro, Tanzania revealed that manure management practices were different from traditional practices mainly due to lack of land. Manure was collected and conveyed by using tools by 94% of respondents, while others used water or bare hands. Seventy six percent of respondents collected manure from animal houses at least once a day, a feature that was associated with housing characteristics (p<0.05). Heaping was a common manure storage method although other cattle keepers directly spread manure on land. Manure was disposed of within residential area by 70% of respondents and this practice was associated with land area owned by or under control of the households (p<0.05). The current manure management practices did not protect either humans, animals or the environment against the risk of contamination with potential zoonotic pathogens and therefore there is a need for the formulation of guidelines on safe manure management practices.
Factors associated with acquisition of enteric episodes in cattle waste handlers in Morogoro, Tanzania
(Research Gate, 2017) Madoshi, B. P.; Lupindu, A. M.; Mtambo, M. M. A.; Muhairwa, A. P.; Olsen, J. E.
The study explored risk factors for acquiring enteric disease in animal waste handlers as occupational hazards. A qualitative survey of 124 of respondents from Morogoro peri-urban and urban areas was done. Eight four respondents had experienced enteric episodes as compared to forty who didn’t mention, and among those who mentioned the episodes, 55.95% had reported this to a health facility while 44.05% had consulted a nearby pharmacy or drug shop. Heaping in living plots was mostly practiced storage method within living plots (70.97 %). The percentage of handlers who were aware of risks for acquiring enteric pathogens from cattle after handling their wastes was 43.55%. There was limited awareness of government guideline on handling such wastes (3.23%) and washing hands without soap was found to be the most common health measures taken after handling animal wastes (70.16%). Statistically significant difference was observed in knowledge on occupational hazards (p = 0.001), zoonotic pathogens awareness (p=0.05), experience of participants (p=0.05) and protective gears (p = 0.022). The animal wastes handlers could be constantly exposed to risks associated with enteropathogens due to the lack of training on proper measures to handle animal wastes as occupational hazard, government interventions of formulating laws and by-laws which are user friendly. This could protect the attendants themselves against the zoonotic enteropathogens, the public in general and the environment.
First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania
(Mary Ann Liebert, Inc., 2018-04-01) Katakweba, A. A. S.; Muhairwa, A. P.; Lupindu, A. M.; Damborg, P.; Rosenkrantz, J. T.; Minga, U. M.; Mtambo, M. A.; Olsen, J. E.
This study provides an estimate of antimicrobial resistance in intestinal indicator bacteria from humans (n = 97) and food animals (n = 388) in Tanzania. More than 70% of all fecal samples contained tetracycline (TE), sulfamethoxazole (STX), and ampicillin (AMP)-resistant coliforms, while cefotaxime (CTX)-resistant coliforms were observed in 40% of all samples. The average Log10 colony forming units/g of CTX-resistant coliforms in samples from humans were 2.20. Of 390 Escherichia coli tested, 66.4% were resistant to TE, 54.9% to STX, 54.9% to streptomycin, and 36.4% to CTX. Isolates were commonly (65.1%) multiresistant. All CTX-resistant isolates contained blaCTX-M gene type. AMP- and vancomycin-resistant enterococci were rare, and the average concentrations in positive samples were low (log10 0.9 and 0.4, respectively). A low-to-moderate resistance (2.1–15%) was detected in 240 enterococci isolates to the drugs tested, except for rifampicin resistance (75.2% of isolates). The average number of sulII gene copies varied between Log10 5.37 and 5.68 with no significant difference between sample source, while cattle had significantly higher number of tetW genes than humans. These findings, based on randomly obtained samples, will be instrumental in designing antimicrobial resistance (AMR) intervention strategies for Tanzania
Isolation of vancomycin-resistant Enterococcus from apparently healthy human animal attendants, cattle and cattle wastes in Tanzania
(Wiley Online Library, 2018-03-07) Madoshi, B. P.; Mtambo, M. M. A.; Muhairwa, A. P.; Lupindu, A. M.; Olsen, J. E.
AIM: The study aimed to isolate and characterize Enterococcus species from apparently healthy waste attendants, cattle and cattle waste in Tanzania. Emphasis was given to antimicrobial resistance and in particular occurrence of vancomycin (VA)-resistant enterococci. METHODS AND RESULTS: Faecal samples were collected from healthy cattle, cattle waste attendants and cattle house wastes, and isolation of Enterococcus species was performed using Slanetz Bartley agar. Isolates were characterized with regard to species, antimicrobial susceptibility and presence of VA resistance genes. Enterococcus faecalis was the most prevalent species from all sources of isolation (43·5%), followed by Enterococcus faecium (38·4%). Isolates of E. faecium showed a higher number of phenotypic antimicrobial resistance than isolates of E. faecalis. Fifty-eight isolates, which showed resistance or intermediate resistance to VA by disc diffusion test, were analysed for VA-resistant Enterococcus (VRE) by PCR. The vanA gene was detected in 14 isolates of E. faecium and 12 isolates of E. faecalis, while vanB was detected in three isolates. No isolates were found to carry vanC1-gene. CONCLUSION: VRE was detected in both human and cattle samples, despite no known use of antimicrobial agents that can select for VRE in livestock in Tanzania. Enterococcus faecalis was the most commonly isolated species from cattle and humans. SIGNIFICANCE AND IMPACT OF THE STUDY: The study provides information on the prevalence of VRE in human and nonhuman samples in Tanzania calling for further studies on the origin of VRE in such isolates, since no selection mechanism in Tanzania are known.
Marketing of free range local chickens in Dar Es Salaam City: Some implications for Rural Extension Services in Tanzania
(Sokoine University of Agriculture, 2003) Mlozi, M. R.S.; Mtambo, M. M.; Olsen, J. E.
This study was conducted from 29th April, 2003 to 6th May, 2003 with the main aim of of the study was to investigate the marketing strategies and the income that village buyers and the urban middle-persons earned from the free-range local chicken (FRLCs), i.e. the latter bought FRLCs in the rural villages and the former sold them in the city of Dar es Salaam. The study interviewed 160 respondents involved in the marketing of FRLC, and of these, 88 were village buyers and 72 were middle-persons. The village buyers went out in the rural areas, bought FRLCs and transported them to Dar es Salaam city and sold chicken to the middle-persons who in turn sold them to urban consumers. To cover the 13 markets scattered in the three Districts (Kinondoni, Ilala, Temeke) in the region of Dar es Salaam, three trained enumerators were temporarily employed to interview village buyers and urban middle-persons using a pre-tested and validated questionnaires. The study found that most the people involved in the FRLCs business were younger (30 to 45 years of age) and over 90 percent were males. Tuesdays and Thursdays were the days when most of the FRLCs were brought in from the up-country destinations. Most of the FRLCs sold in 13 markets in the city of Dar es Salaam originated from Dodoma and Singida regions—further proving earlier proof that these two regions produced the most FRLCs sold in the city. Most of the FRLCs were transported on trains and that the tenga was the most common container used for transporting chicken to the markets. Cocks were priced highly than hens, and that August, September and October FRLCs fetched high prices. Respondents showed that most of the FRLCs showed disease symptoms in September. The urban middle-persons earned more profits from selling FRLCs than did the village buyers. There are a number of implications for rural extension services that emanate from this study that could improve the husbandry of FRLCs, hence, contributing to household income and poverty alleviation in rural areas. Furthermore, the findings of this study have demonstrated the enormous potential of FRLCs a resource that few have tapped leaving out the smallholder to get a pittance. Therefore, this study makes recommendations at six levels of operation: the extension officers, the researchers, the village buyers and urban middle-persons, the NGOs and the Government.
Occurrence and characterization of Shiga toxin-producing Escherichia coli o157:h7 and other non-sorbitol–fermenting e. coli in cattle and humans in urban areas of Morogoro, Tanzania
(Mary Ann Liebert, Inc., 2014) Lupindu, A. M.; Olsen, J. E.; Ngowi, H. A.; Msoffe, P. L. M.; Mtambo, M. M.; Scheutz, F.; Dalsgaard, A.
Escherichia coli strains such as Shiga toxin–producing E. coli (STEC), enteropathogenic E. coli, enterotoxigenic, attaching, and effacing E. coli, and enteroinvasive E. coli cause diarrhea in humans. Although other serotypes exist, the most commonly reported STEC in outbreaks is O157:H7. A cross-sectional study was conducted to isolate and characterize non-sorbitol–fermenting (NSF) E. coli O157:H7 from urban and periurban livestock settings of Morogoro, Tanzania. Human stool, cattle feces, and soil and water samples were collected. Observations and questionnaire interview studies were used to gather information about cattle and manure management practices in the study area. E. coli were isolated on sorbitol MacConkey agar and characterized by conventional biochemical tests. Out of 1049 samples, 143 (13.7%) yielded NSF E. coli. Serological and antimicrobial tests and molecular typing were performed to NSF E. coli isolates. These procedures detected 10 (7%) pathogenic E. coli including STEC (n=7), enteropathogenic E. coli (EPEC) (n=2), and attaching and effacing E. coli (A/EEC) (n=1) strains. The STEC strains had the ability to produce VT1 and different VT2 toxin subtypes that caused cytopathic effects on Vero cells. The prevalence of STEC in cattle was 1.6%, out of which 0.9% was serotype O157:H7 and the overall prevalence of diarrheagenic E. coli in cattle was 2.2%. The serotypes O157:H7, O142:H34, O113:H21, O+:H-,O +:H16, and O25:H4 were identiﬁed. One ESBLproducing isolate showed the MLST type ST131. To our knowledge, this is the ﬁrst ﬁnding in Tanzania of this recently emerged worldwide pandemic clonal group, causing widespread antimicrobial-resistant infections, and adds knowledge of the geographical distribution of ST131. Cattle manure was indiscriminately deposited within residential areas, and there was direct contact between humans and cattle feces during manure handling. Cattle and manure management practices expose humans, animals, and the environment to pathogenic E. coli and other manure-borne pathogens. Therefore, there is a need to improve manure management practices in urban and periurban areas to prevent pathogen spread and associated human health risks.
spa typing and antimicrobial resistance of staphylococcus aureus from healthy humans, pigs and dogs in Tanzania
(The Journal Infection Developing Countries, 2016-07) Katakweba, A. S.; Muhairwa, A. P.; Espinosa -Gongora, C.; Guardabassi, L.; Mtambo, M. A.; Olsen, J. E.
Introduction: Staphylococcus aureus is an opportunistic pathogen causing infections in humans and animals. Here we report for the first time the prevalence of nasal carriage, spa typing and antimicrobial resistance of S. aureus in a Tanzanian livestock community. Methodology: Nasal swabs were taken from 100 humans, 100 pigs and 100 dogs in Morogoro Municipal. Each swab was enriched in Mueller Hinton broth with 6.5% NaCl and subcultured on chromogenic agar for S. aureus detection. Presumptive S. aureus colonies were confirmed to the species level by nuc PCR and analysed by spa typing. Antimicrobial susceptibility patterns were determined by disc diffusion method. Results: S. aureus was isolated from 22 % of humans, 4 % of pigs and 11 % of dogs. A total of 21 spa types were identified: 13, 7 and 1 in human, dogs, and pigs, respectively. Three spa types (t314, t223 and t084) were shared between humans and dogs. A novel spa type (t10779) was identified in an isolate recovered from a colonized human. Antimicrobials tested revealed resistance to ampicillin in all isolates, moderate resistances to other antimicrobials with tetracycline resistance being the most frequent. Conclusion: S. aureus carrier frequencies in dogs and humans were within the expected range and low in pigs. The S. aureus spa types circulating in the community were generally not shared by different hosts and majority of types belonged to known clones. Besides ampicillin resistance, moderate levels of antimicrobial resistance were observed irrespective of the host species from which the strains were isolated.
Transmission of antibiotic-resistant Escherichia coli between cattle, humans and the environment in peri-urban livestock keeping communities in Morogoro, Tanzania
(ELSEVIER, 2014-07-23) Lupindu, A. M.; Dalsgaard, A.; Msoffe, P. L.; Ngowi, H. A.; Mtambo, M. M.; Olsen, J. E.
Urban and peri-urban livestock farming is expanding world-widely because of increased urbanization and demands for food of animal origin. Such farming practices pose a public health risk as livestock are reservoirs of several zoonotic pathogens. In an attempt to determine the fecal transmission between livestock and people, 100 household clusters keeping cattle in close proximity of humans were selected in urban and peri-urban areas of Morogoro in Tanzania. One hundred eighteen ampicillin and tetracycline resistant Escherichia coli (40 from human stool, 50 from cattle feces, 21 from soil and seven from water samples) were isolated from 44 different clusters. Pulsed-field gel electrophoresis (PFGE) of XbaI digested chromosomal DNA was used to compare the genetic relatedness of the ampicillin- and tetracycline-resistant E. coli isolates. Indistinguishable PFGE band patterns of the ampicillin- and tetracycline-resistant E. coli isolates were found in samples from 23 (52%) clusters. This suggests that transfer of fecal microorganisms between cattle, humans, water and soils within the farms and from livestock farms to the neighborhood occurred commonly. Logistic regression showed that animal housing infrastructures (Odd Ratio=11.2, 95% CI=1.1-119.3) were associated with E. coli showing identical PFGE types within and between clusters. There is a need to improve animal husbandry and manure management practices to reduce risks of transmission of enteropathogens between livestock and humans in urban and peri-urban farming.