Virulence pattern of circulating aeromonads isolated from farmed nile tilapia in Tanzania and novel antibiotic free attenuation of aeromonas hydrophila strain tzr7-2018
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Aquaculture Reports
Abstract
Aeromonads are gram-negative, rod-shaped, facultative anaerobes bacteria known to cause motile aeromonads
septicemia diseases (MAS) in warm freshwater farmed fish. Outbreaks are associated with pathogenicity of
aeromonads in fish which is partly contributed by virulence characteristics of the etiological agent. The objective
of this study was to assess the virulence characteristics of the previously isolated and identified aeromonads, and
attenuate potential Aeromonas hydrophila strain TZR7-2018 to serve as local vaccine candidate. Six virulence
genes and other virulence characteristics were molecularly and phenotypically assessed both using in-vitro and
in-vivo approaches. Attenuation of A. hydrophila parent strain TZR7-2018 + was performed by passaging through
thermal continuous sub-culturing 40 times in Tryptic soy agar (TSA). Bacterin was prepared by formalin in-
activation from the same parent strain. Humoral responses were assayed using quantitative serological agglu-
tination test (qSAT) while protective efficacy was measured through relative percent survival (RPS). A total 240
Nile tilapia fingerlings with an average weight of 8.1 ± 0.4 g were used in all in-vivo studies. The presence of
aerolysin (aer), cytotoxic enterotoxin (act), elastase (ahy), haemolysin (hly), serine (ser) and polar flagella (fla)
genes were determined using PCR. Out of 201 isolates, 75.1 % (151/201) of the aeromonads possessed virulence
genes (120=A. hydrophila and 31=Aeromonas veronii). The virulence gene pattern of aer/hly/fla was the most
prominent with the prevalence of 12.6 %. The attenuated strain TZR7-2018 − showed reduced: colon size,
multiplication rate, cell size and loss in; haemolysis, motility and capsule. Humoral responses increased gra-
dually and reached maximum at day 28 in both attenuated and bacterin formulation given through in-
traperitoneal (IP) injection and immersion (IM). A RPS of 82.3 %, 71.4 % and 85.1 %, were recorded to the
attenuated vaccine given through IP and IM and bacterin provided through IP respectively.
Therefore the attenuated strain TZR7-2018 − obtained through thermal continuous subculture technique and
the bacterin proved to be efficacious and can serve as vaccine candidate.
Description
Journal article
Keywords
Virulence, Attenuation, Aeromonas spp, Heat-shock, Farmed tilapia, Tanzania
Citation
https://doi.org/10.1016/j.aqrep.2020.100300