Genetic characterization and antimicrobial susceptibility patterns of proteus mirabilis isolated from domestic rats in Arusha municipaltiy, Tanzania
| dc.contributor.author | Ndakidemi, Floramanka Patrick | |
| dc.date.accessioned | 2023-02-10T12:09:46Z | |
| dc.date.available | 2023-02-10T12:09:46Z | |
| dc.date.issued | 2022 | |
| dc.description | Dissertation | en_US |
| dc.description.abstract | The aim of this study was to genetically characterize and assess antimicrobial susceptibility patterns of Proteus mirabilis isolates from rats cohabiting with human dwellings. A cross sectional survey was conducted where a total of 139 rats were trapped in houses and around peri domestic areas in selected wards in Arusha city. Following euthanization, rats were identified to Genus/species level using morphological and morphometric features. Deep intestinal swabs were ascetically obtained and pre enriched in buffered peptone water prior laboratory analysis conventional culture methods and biochemical methods were used for bacterial isolation. Molecular confirmation of the isolates was done using the 16s ribosomal RNA PCR identification method. Susceptibilities to Tetracycline (TE, 30μg), Trimethoprim sulfamethoxazole (SXT, 25 μg), Ciprofloxacin (CIP, 5μg), Cefotaxime (CTX,5μg), Ampicilin (AMP,10μg), Azithromycin (AZM, 15μg) and Gentamycin (CN,10μg) was performed and resistance genes (bla TEM, tetA, tetB, mphA, SHV, bla CTX-M, sul1 and sul2) were traced in each isolate using PCR methods. Mixed rat species Rattus rattus (55.4%; n=77) Mus musculus (15.8%; n=22) and Mastomys natalensis (28.8%; n=40) were captured. Proteus mirabilis was isolated from 4 samples (2%) from Rattus rattus. All isolates were 100% similar to P. mirabilis strains from NCBI. Constructed phylogenetic tree showed all P. mirabilis isolated from this study were closely related to Tunisia isolates. Three isolates showed MDR trait against Triomethoprim- sulfamethaxole, Azithromycin, and Ampicillin., all isolates were resistance to Azithromycin, and Ampicillin, three were resistance to Triomethoprim- sulfamethaxole, and intermediate to Tetracycline and all susceptible to Ciprofloflaxcin, Gentamicin and Cefotaxime, PCR analysis showed the presence of TetA, blaTEM, Sul1 and Sul2 resistant genes in all isolates. The study has shown that rats are potential reservoirs of Proteus mirabilis with antimicrobial resistance trait that could be transmitted to the public and it’s the first study to isolate P. mirabilis from rats in Tanzania with antimicrobial resistance trait. | en_US |
| dc.description.sponsorship | African Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development (ACE IRPM and BTD), | en_US |
| dc.identifier.uri | http://www.suaire.sua.ac.tz/handle/123456789/4927 | |
| dc.language.iso | en | en_US |
| dc.publisher | Sokoine University of Agriculture | en_US |
| dc.subject | Genetic characterization | en_US |
| dc.subject | Antimicrobial susceptibility patterns | en_US |
| dc.subject | Proteus mirabilis isolated | en_US |
| dc.subject | Domestic rats | en_US |
| dc.subject | Tanzania | en_US |
| dc.subject | Arusha municipality | en_US |
| dc.title | Genetic characterization and antimicrobial susceptibility patterns of proteus mirabilis isolated from domestic rats in Arusha municipaltiy, Tanzania | en_US |
| dc.type | Thesis | en_US |