Susceptibility of campylobacter strains to selected natural products and frontline antibiotics
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Campylobacter species have developed resistance to existing antibiotics. The development of
alternative therapies is, therefore, a necessity. This study evaluates the susceptibility of Campylobacter
strains to selected natural products (NPs) and frontline antibiotics. Two C. jejuni strains (ATCC®
33560TM and MT947450) and two C. coli strains (ATCC® 33559TM and MT947451) were used.
The antimicrobial potential of the NPs, including plant extracts, essential oils, and pure phytochemicals,
was evaluated by broth microdilution. The growth was measured by spectrophotometry and
iodonitrotetrazolium chloride. Antibiotic resistance genes (tet(O) and gyrA) were characterized at
the molecular level. The minimum inhibitory concentrations (MICs) and the minimum bactericidal
concentrations (MBCs) ranged from 25 to 1600 μg/mL. Cinnamon oil, (E)-Cinnamaldehyde, clove oil,
eugenol, and baicalein had the lowest MIC and MBC values (25–100 μg/mL). MT947450 and MT947451
were sensitive to erythromycin and gentamicin but resistant to quinolones and tetracycline. Mutations
in gyrA and tet(O) genes from resistant strains were confirmed by sequencing. The findings show that
NPs are effective against drug-sensitive and drug-resistant Campylobacter strains. The resistance to
antibiotics was confirmed at phenotypic and genotypic levels. This merits further studies to decipher
the action mechanisms and synergistic activities of NPs
Description
Journal article
Keywords
Antibiotics, Campylobacter, Plant extracts, Essential oils, Phytochemicals, Resistance
Citation
doi:10.3390/antibiotics9110790