Challenges in determining the pathogenicity status of Leptospira isolates with phenotypic methods: The need for a polyvalent approach
| dc.contributor.author | Mgode, G. F. | |
| dc.contributor.author | Machang’u, R. S. | |
| dc.contributor.author | Collares-Pereira, M. | |
| dc.contributor.author | Vieira, M. L. | |
| dc.contributor.author | Goris, M. G. A. | |
| dc.contributor.author | Engelbert, M. | |
| dc.contributor.author | Hartskeerl, R. A. | |
| dc.date.accessioned | 2017-04-19T07:10:23Z | |
| dc.date.available | 2017-04-19T07:10:23Z | |
| dc.date.issued | 2010-12-04 | |
| dc.description | African Journal of Microbiology Research, 2010; 4 (23): 2528-2533 | en_US |
| dc.description.abstract | Understanding the pathogenic status of leptospires, the causative agents of leptospirosis, is important for successful laboratory diagnosis and control programmes of this zoonosis. Leptospires are difficult to differentiate morphologically; therefore, their pathogenic, intermediate or saprophytic status is currently determined based on both phenotypic tests like growth response in medium containing 8-azaguanine and growth at low temperature (13°C), and genotypic methods. The present study reports on the pathogenic versus saprophytic characterization of nine Leptospira isolates from animal hosts (rodents and dogs) and humans, with specific interest on a canine isolate coded “Dog109”, which showed an ambiguous or intermediate status according to conventional (phenotypic) and molecular (genotypic) tests. The results strongly indicate the need of a polyvalent analytical approach for improving the differentiation of the pathogenic status of circulating serovars, | en_US |
| dc.identifier.issn | 1996-0808 | |
| dc.identifier.uri | https://www.suaire.sua.ac.tz/handle/123456789/1443 | |
| dc.language.iso | en | en_US |
| dc.subject | Characterization | en_US |
| dc.subject | Isolates | en_US |
| dc.subject | Leptospira | en_US |
| dc.subject | Saprophytic | en_US |
| dc.title | Challenges in determining the pathogenicity status of Leptospira isolates with phenotypic methods: The need for a polyvalent approach | en_US |
| dc.type | Article | en_US |