Morphological and molecular diversity of stinging nettle (Urtica simensis) from northern Ethiopia
| dc.contributor.author | Abdulkadir, B. E | |
| dc.date.accessioned | 2021-06-26T05:57:32Z | |
| dc.date.available | 2021-06-26T05:57:32Z | |
| dc.date.issued | 2020 | |
| dc.description | Masters Thesis | en_US |
| dc.description.abstract | Urtica simensis is an erect perennial herb among the species of nettle which belongs to the family Urticaceae that is endemic to Ethiopia and locally known as Samma. It is cooked and consumed as vegetable in some parts of Ethiopia. It has medicinal properties where traditionally people use it in treating different types of diseases, including infectious diseases like allergies, diabetes and Malaria. This study was conducted to evaluate the diversity of Urtica simensis to address and fill the gap on its genetic variability and distribution using morphological characters and ISSR DNA markers. A total 133 plant samples were collected from Northern growing areas of Ethiopia (Gondar and Mekelle), based on variations in morphological characters. Morphological characterization was conducted on six morphological traits which demonstrated variation based on PCA and correlation matrix analysis. Molecular characterization was carried out using ISSR markers where, only 5 were selected out of 16 ISSR primers that produced a total of 445 scorable bands and 30 polymorphic loci, 100% of polymorphic bands, 1.88 genetic diversity and 2.75 Shannon index from a collection of 133 plant samples. Highest genetic diversity (H) was found to be 0.4286 and Shannon Information index (I) was 0.6197. The highest genetic diversity was indicated in plant samples from Mekelle compared to samples from Gondar (0.3462 and 0.3152 respectively). The Dendrogram based on Jaccard’s similarity coefficients generated by UPGMA cluster analysis using morphological and ISSR data shows major and minor clusters with broad distribution of Urtica simensis individuals over the entire tree which indicates the low divergence in morphological appearance among populations from both study areas. Based on the results of this study, morphological and ISSR markers were effective in studying genetic diversity of Urtica simensis demonstrated by variations in terms of morphological appearances and genetic variability. These results have valuable effect on characterization of Urtica simensis genetic resources in different parts of Ethiopia for conservation purposes. | en_US |
| dc.description.sponsorship | IUCEA | en_US |
| dc.identifier.uri | https://www.suaire.sua.ac.tz/handle/123456789/3620 | |
| dc.language.iso | en | en_US |
| dc.publisher | Sokoine University of Agriculture | en_US |
| dc.subject | Morphological | en_US |
| dc.subject | Molecular diversity | en_US |
| dc.subject | Northern Ethiopia | en_US |
| dc.title | Morphological and molecular diversity of stinging nettle (Urtica simensis) from northern Ethiopia | en_US |
| dc.type | Thesis | en_US |