Prevalence, characterisation and antimicrobial resistance profiles of salmonella isolates from healthy broiler and indigenous free range chickens in Morogoro, Tanzania

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dc.contributor.authorMunuo, Lidia Abiael
dc.date.accessioned2022-02-22T08:53:00Z
dc.date.available2022-02-22T08:53:00Z
dc.date.issued2021
dc.descriptionDissertationen_US
dc.description.abstractThe study was conducted to determine prevalence, biochemical profiles and antimicrobial susceptibility profiles of non typhoidal salmonella (NTS) in indigenous free range and broiler chickens in Morogoro Municipality. Between November 2019 and May 2020, a total of 384 cloaca swab samples from Magadu, Mzinga and Bigwa wards were collected. Identification was done by standard bacteriological methods, serotyping and genetically confirmed by PCR using Salmonella specific primers pair and Salmonella enterica primer pair (invA and iroB gene primers). Antimicrobial sensitivity tests were done using Ampicillin, Cefaclor, Imipenem, Gentamycin, Ciprofloxacin, Sulfamethaxazole- Trimethoprim and Tetracycline antimicrobial discs. Out of 384 samples, 11 (2.9%) samples confirmed to be Salmonella of which 8(4%) were from broilers and 3(1.6%) were from free range chickens. Of the 11 isolates 8 were from group B and 3 isolates were from group D. Bigwa ward showed high prevalence (5.2%) of Salmonella than the other wards, broilers being the more prevalent in Salmonella than free range chickens. Antimicrobial susceptibility results showed variable level of sensitivity to majority of antimicrobial tested, however, variable level of resistance were also found with 7 isolates resistant to Ampicillin, 4 isolates resistant to Sulfamethoxazole-Trimethoprim and 3 isolates resistant to Tetracycline. Screening for resistant genes detected three isolates with Sulfamethaxole (sulII) resistant gene and none for Tetracycline and Ampicillin. This study revealed the presence of Salmonella carriers among chicken kept in Morogoro Municipal with antimicrobial resistances from both free range and broilers chickens. The results underline the importance of the biosecurity measures in the production and processing of chicken for human consumption, Similarly improvement of management is recommended to stop transmission of Salmonella from natural carriers to chickens as indicated by faecal carriers found. Contamination or spread from rats that are natural carriers to poultry needs to be further investigated.en_US
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/3930
dc.language.isoenen_US
dc.publisherSokoine University of Agricultureen_US
dc.subjectSalmonellaen_US
dc.subjectAntimicrobial resistanceen_US
dc.subjectIndigenous free range chickensen_US
dc.subjectHealthy broileren_US
dc.titlePrevalence, characterisation and antimicrobial resistance profiles of salmonella isolates from healthy broiler and indigenous free range chickens in Morogoro, Tanzaniaen_US
dc.typeThesisen_US

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