Activity of sterols isolated from the leaves of commiphora swynnertonii (Burtt) against some nosocomial infectious bacteria
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Date
2022-09-04
Journal Title
Journal ISSN
Volume Title
Publisher
The Journal of Phytopharmacology
Abstract
Commiphora swynnertonii (Burtt) is traditionally used for medicinal purposes both in humans and
animals in Tanzania. Previously, its leaves extract has been demonstrated antibacterial activity.
However, there is limited information on identification of pure compounds responsible for its observed
antibacterial activity. Therefore, this study was conducted to isolate and evaluate antibacterial activity of
pure compounds isolated from its leaves extract. Colum chromatographic separation of its
dichloromethane leaves extract afforded the isolation of two sterols namely, Cholest-7-en-3β-ol (1) and
β-sitosterol (2). Their structures were deduced by using NMR data experimentally obtained and
comparison with spectral data available in literatures. The sterols were evaluated for activity against four
nosocomial infectious bacteria namely; Staphylococcus aureus, Enterococcus faecalis, Escherichia coli
and Pseudomonas aeruginosa to determine Minimum Inhibitory Concentrations (MIC) by broth
microdilution method. Cholest-7-en-3β-ol (1) demonstrated strong activity against S. aureus (MIC= 0.5
mg/ml) and weak activities (MIC >2mg/ml) against other bacteria. β-sitosterol (2) exhibited weak
activities (MIC >2mg/ml) against all tested bacteria. Therefore, presence of antibacterial active
compound(s) such as Cholest-7-en-3β-ol (1) among others in the leaves of C. swynnertonii can justify its
earlier reported antibacterial activity and further confirms the claimed traditional uses of this plant in the
treatment of bacterial infections.
Description
Research article
Keywords
β-sitosterol, Burseraceae, Cholest-7-en-3β-ol, Commiphora swynnertonii, Nosocomial Infectious Bacteria, Sterols