Drug susceptibility profiles and factors associated with non-tuberculous mycobacteria species circulating among patients diagnosed with pulmonary tuberculosis in Tanzania

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dc.contributor.authorMaya, Togolani Godfrey
dc.contributor.authorKomba, Erick Vitus
dc.contributor.authorMensah, Gloria Ivy
dc.contributor.authorMbelele, Peter Masunga
dc.contributor.authorMpagama, Stella George
dc.contributor.authorMfinanga, Sayoki Godfrey
dc.contributor.authorAddo, Kennedy Kwasi
dc.contributor.authorKazwala, Rudovick Reuben
dc.date.accessioned2025-11-05T08:57:58Z
dc.date.available2025-11-05T08:57:58Z
dc.date.issued2022
dc.descriptionJournal article
dc.description.abstractBackground While most Non-tuberculous mycobacteria (NTM) are saprophytic, several species have been associated with human diseases, from localized infection to disseminated diseases. Pulmonary NTM infections lead to TB-like disease called NTM pulmonary disease (NTM- PD). Due to variation in treatment options among NTM species, it is necessary to identify the species and determine drug susceptibility profiles to inform the choice of appropriate regimen for the disease. Design A total of 188 culture-positive isolates from patients diagnosed with TB were screened for NTM at the Central Tuberculosis Reference Laboratory. All NTM were further speciated using GenoType® Mycobacterium—Common Mycobacterium and Additional species (GenoType® CM/AS) kit. Mycobacteria avium complex (MAC) and Mycobacteria absces- sus complex (MABC) which could not be identified with the test to species were subjected to GenoType® Mycobacteria NTM-DR for further speciation. Using the same test, identified MAC and MABC were genotyped to determine the drug susceptibility profile for each isolate to macrolide and aminoglycosides. Results Of all isolates identified as mycobacteria, 24 (13%) were NTM. Fifteen isolates could be identified to species level of which prevalent species was M. avium sub. intracellulare 4 (27%). A total of 10 isolates were MAC (n = 6) and MABC (n = 4) were subjected to Geno- Type® Mycobacteria NTM-DR for determination of macrolide and aminoglycoside suscepti- bility. Three of the four MABC had a mutation at the T28 position of the erm (41). All MAC were susceptible to both drugs. Conclusion In this study, MAC was the most frequently isolated NTM species followed by MABC. While all MAC and MABC identified, were susceptible to aminoglycosides, three MABC were resistant to the macrolides due to mutation at position 28 of the erm (41) gene. For this, it is important for clinicians need to rule out NTM, understand species and their drug susceptibil- ity for optimal case management.
dc.description.sponsorshipConsortium of donor including the African Academy of Sciences (AAS) Alliance for Accelerating Excellence in Science in Africa (AESA), the New Partnership for Africa’s Development Planning and Coordinating (NEPAD) Agency, the Wellcome Trust [107753/A/15/Z] and the UK government.
dc.identifier.citationhttps://doi.org/10.1371/journal. pone.0265358
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/20.500.14820/7118
dc.language.isoen
dc.publisherPLoS ONE
dc.subjectTB
dc.subjectTB-Tanzania
dc.titleDrug susceptibility profiles and factors associated with non-tuberculous mycobacteria species circulating among patients diagnosed with pulmonary tuberculosis in Tanzania
dc.typeArticle

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