Morphological changes in the sperm storage tubules of the japanese quail exposed to methy-2- benzimidazole carbamate
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Date
2016
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Anatomy Journal of Africa
Abstract
The current investigation was an attempt to establish the effect of various doses of methyl-2-
benzimidazole carbamate (carbendazim®) on the morphology of the Sperm Storage Tubules (SST)
in the Japanese quail (Coturnix coturnix japonica). Carbendazim® in sunflower oil base was
administered orally at doses of 0mg/kg (control), 25mg/kg, 100mg/kg, 400mg/kg and 800mg/kg
body weight. Tissue samples from Uterovaginal junction were processed for both light (LM) and
Transmission electron (TEM) microscopic study following standard procedures. The result showed
that, at LM level, no histopathological changes were observed at a dose of 25mg/kg b.w.t. A
significant decrease in SST width and luminal diameters was observed at doses of 100mg/kg and
400mg/kg b.w.t (p < 0.05). In addition, doses of 400mg/kg and 800mg/kg b.w.t caused leukocytic
infiltration and hyperaemia in the lamina propria-submucosa. At these doses SST were devoid of
spermatozoa. TEM results showed pyknosis, swollen mitochondria, vacuolation and increased number
of lysosomes in degenerating SST. The observed morphological changes indicate the ability of
carbendazim to disrupt structural integrity of SST as well as its storage capacity. This poses a great
threat to the fertility of exposed birds and thus care must be taken to reduce environmental
contamination.
Description
Journal Article
Keywords
Carbendazim, Histopathology, Ultrastructure, Sperm storage tubules, Japanese quail