Supplementation with quaternary benzo(c)phenanthridine alkaloids decreased salivary cortisol and salmonella shedding in pigs after transportation to the slaughterhouse
| dc.contributor.author | Artuso-Ponte, V. | |
| dc.contributor.author | Moeller, S. | |
| dc.contributor.author | Rajala-Schultz, P. | |
| dc.contributor.author | Medardus, J. J. | |
| dc.contributor.author | Munyalo, J. | |
| dc.contributor.author | Lim, K. | |
| dc.contributor.author | Gebreyes, W. A. | |
| dc.date.accessioned | 2020-08-25T10:31:37Z | |
| dc.date.available | 2020-08-25T10:31:37Z | |
| dc.date.issued | 2015 | |
| dc.description | FOODBORNE PATHOGENS AND DISEASE, Volume 12, Number 11, 2015 | en_US |
| dc.description.abstract | The present study was aimed at evaluating the effect of herbal extracts supplementation, particularly quaternary-benzo(c)phenanthridine alkaloids (QBA), which have been previously demonstrated to have anti- inflammatory, antimicrobial, and immune-modulator effects. We investigated the role of QBA on stress re- sponse and Salmonella shedding in finishing pigs transported to the slaughterhouse. A total of 82 pigs were orally challenged with a Salmonella cocktail (day 0) containing Salmonella Meleagridis, Hartford, Bo- vismorbificans and Newport serovars and randomly assigned to three treatment groups after 2 wks (day [D] 14): T1, in-feed QBA; T2, in-feed and water-soluble QBA; CON, nonsupplemented). Pigs were transported to the slaughterhouse 2 weeks after intervention (D 28) and slaughtered after nearly 19 h (D 29). Saliva, fecal samples, and carcass swabs were collected from all pigs. Salivary cortisol, Salmonella shedding, and carcass contami- nation were measured. A high positive correlation (Spearman rank correlation coefficient range 0.82–0.93) between salivary cortisol and Salmonella shedding was found after transportation in all groups ( p < 0.05). Only the CON group showed an increase in salivary cortisol after transportation (5.48 ng/mL; p < 0.0001) to con- centrations that were higher than in T1 (2.73 ng/mL; p = 0.0002) and T2 (1.88 ng/mL; p < 0.0001). Salmonella prevalence and shedding decreased after transportation in pigs receiving the QBA intervention ( p < 0.05), whereas the control group showed a significant increase in Salmonella shedding after transportation ( p = 0.04). At D 28, pigs in T2 shed lower numbers of Salmonella as compared to T1 (1.3E + 02 CFU/mL versus 8E + 03 CFU/mL; p = 0.002). Additionally, carcass contamination by Salmonella was higher in the CON group than the treated groups ( p = 0.01). The findings show QBA intervention was effective in reducing transportation stress of pigs, resulting in reduced Salmonella shedding and positively impacting animal welfare and pork safety. | en_US |
| dc.identifier.uri | https://www.suaire.sua.ac.tz/handle/123456789/3157 | |
| dc.language.iso | en | en_US |
| dc.publisher | Mary Ann Liebert, Inc. | en_US |
| dc.relation.ispartofseries | FOODBORNE PATHOGENS AND DISEASE;Volume 12, Number 11 | |
| dc.subject | Quaternary benzo | en_US |
| dc.subject | Alkaloids decreased | en_US |
| dc.subject | Salivary cortisol | en_US |
| dc.subject | Salmonella shedding | en_US |
| dc.subject | Pork safety | en_US |
| dc.title | Supplementation with quaternary benzo(c)phenanthridine alkaloids decreased salivary cortisol and salmonella shedding in pigs after transportation to the slaughterhouse | en_US |
| dc.type | Article | en_US |
| dc.url | 10.1089/fpd.2015.2009 | en_US |