Browsing by Author "Gabriel, Harrison Sadiki"

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    Evaluation of multiplex pcr for detection of mycobacteria tuberculosis complex microorganisms isolated from cattle tissues and human tuberculosis cases Tanzania
    (Sokoine University of Agriculture, 2010) Gabriel, Harrison Sadiki
    A study on the evaluation of multiplex PCR on detection on Mycobacteria tuberculosis complex was carried on samples obtained from Morogoro district. Specimen collected from abattoir in Morogoro Municipal and human cases from Muhimbili Research Station were analysed at the Faculty of Veterinary Medicine Laboratory by Multiplex PCR. A total of eight nine-pooled lymph nodes samples collected from none tuberculin tested slaughter cattle in the study area-Morogoro abattoir were cultured and Seventy two (80.89%) yielded mycobacteria. Seventeen were identified as M.bovis and three as M.tuberculosis. The remaining fifty two were mycobacteria other than tuberculosis (MOTTs). A total of forty five isolates from human sample were submitted for subculture and thirty eight (84%) were positive for mycobacteria. Twenty five isolated were identified as M.tuberculosis. The remaining thirteen were mycobacteria other than tuberculosis. The IS986 and mtp40 multiplex PCR was able to differentiate M.bovis from M.tuberculosis. The finding of this therefore necessitates the collaboration of veterinary and medical professions to the control transmission of M.bovis infection in human and animal populations.