Evaluation of multiplex pcr for detection of mycobacteria tuberculosis complex microorganisms isolated from cattle tissues and human tuberculosis cases Tanzania
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Date
2010
Authors
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Journal ISSN
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Publisher
Sokoine University of Agriculture
Abstract
A study on the evaluation of multiplex PCR on detection on Mycobacteria tuberculosis
complex was carried on samples obtained from Morogoro district. Specimen collected from
abattoir in Morogoro Municipal and human cases from Muhimbili Research Station were
analysed at the Faculty of Veterinary Medicine Laboratory by Multiplex PCR. A total of
eight nine-pooled lymph nodes samples collected from none tuberculin tested slaughter
cattle in the study area-Morogoro abattoir were cultured and Seventy two (80.89%) yielded
mycobacteria. Seventeen were identified as M.bovis and three as M.tuberculosis. The
remaining fifty two were mycobacteria other than tuberculosis (MOTTs). A total of forty
five isolates from human sample were submitted for subculture and thirty eight (84%) were
positive for mycobacteria. Twenty five isolated were identified as M.tuberculosis. The
remaining thirteen were mycobacteria other than tuberculosis. The IS986 and mtp40
multiplex PCR was able to differentiate M.bovis from M.tuberculosis. The finding of this
therefore necessitates the collaboration of veterinary and medical professions to the control
transmission of M.bovis infection in human and animal populations.
Description
Masters Theses
Keywords
Mycobacteria tuberculosis, Microorganisms, Cattle tissues, Human tuberculosis, Tanzania