Transfer of bruchid resistance into farmer’s preferred dry common bean (phaseolus vulgaris l.) varieties in Tanzania

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dc.contributor.authorThaudensia, Herman
dc.date.accessioned2025-02-06T09:59:44Z
dc.date.available2025-02-06T09:59:44Z
dc.date.issued2012
dc.descriptionMaster of Science in Crop Science
dc.description.abstractThis study was undertaken to transfer bruchid resistance against Acanthoscelides oblectus (Say) and Zabrotes subfasciatus (Boh) conditioned by APA locus and ARC2 genes respectively into four Fanners Preferred Varieties (FPVs) , Kablanket (BSUA 59), Njano Uyole (BSUA 29), Soworo (BSUA 73), Carnadian wonder (BSUA 37) collected from major bean producing regions with geographical and ecological representation of Tanzania’s bean growing areas and five APA locus parents (BTICA 132-4-5, BTICA 132 - 4-1, 1CA 43.3/4-7-5, ICA43.9/10-6-3, ICA 43.9/10-7-2) obtained from interspecific hybrids introgression lines expressing a 33kDa seed storage protein and Arcelin 2 donor parent (2R-41B-20-I) obtained from previously developed inbred lines on Rojo background. Selection of the resistant lines were done using gene specific DNA markers and total seed storage proteins. Seed storage proteins (APA and ARC2) of the developed F3 families from this work were analyzed using one dimensional Sodium dodecyl sulphate polyacrylamide gel electrophoresis (1-DSDS-PAGE) and PCR amplification of the DNA fragments corresponding to the targeted marker size. The generated 86 F3 families from crosses between the four FPVs and APA parent lines were analyzed for the presence of 33Kda APA seed storage proteins. Results indicated that 15 families were homozygous for the presence for APA. The expected band of 33kDa was not transferred in Carnadian wonder (BSUA 37). A total of 100 F3 families were developed from FPVs and ARC2 parents. Results showed that four families two from Canardian (BSUA37) and two from Njano uyole were homozygous for presence of ARC 2 seed storage protein . The Chi square values obtained from each cross with APA and ARC2 agreed with Mendelian ratios implying that both genes were dominant and monogenic inherited. The homozygous lines expressing APA and ARC2 will be subjected to bruchid screening and best for inhibition of bruchid development will be needed as parents for further back crossing breeding
dc.description.sponsorshipThe McK night Foundation
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/6538
dc.language.isoen
dc.publisherSokoine University of Agriculture
dc.subjectDry common bean
dc.subjectPhaseolus vulgaris l.
dc.subjectBean Bruchid Species
dc.subjectBean Varieties
dc.subjectTanzania
dc.titleTransfer of bruchid resistance into farmer’s preferred dry common bean (phaseolus vulgaris l.) varieties in Tanzania
dc.typeThesis

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