Exposure to salinity and light spectra regulates glucosinolates, phenolics, and antioxidant capacity of brassica carinata L. microgreens

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dc.contributor.authorMaina, Sylvia
dc.contributor.authorRyu, Da Hye
dc.contributor.authorCho, Jwa Yeong
dc.contributor.authorJung, Da Seul
dc.contributor.authorPark, Jai-Eok
dc.contributor.authorNho, Chu Won
dc.contributor.authorBakari, Gaymary
dc.contributor.authorMisinzo, Gerald
dc.contributor.authorJung, Je Hyeong
dc.contributor.authorYang, Seung-Hoon
dc.contributor.authorKim, Ho-Youn
dc.date.accessioned2023-08-22T06:08:25Z
dc.date.available2023-08-22T06:08:25Z
dc.date.issued2021-07-26
dc.descriptionJournal articleen_US
dc.description.abstractThe effect of salt treatment on Brassica carinata (BC) microgreens grown under different light wavelengths on glucosinolates (GLs) and phenolic compounds were evaluated. Quantifiable GLs were identified using ultra-high performance-quadrupole time of flight mass spectrometry. Extracts’ ability to activate antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) was evaluated on human colorectal carcinoma cells (HCT116). Furthermore, BC compounds’ ability to activate expression of nuclear transcription factor-erythroid 2 related factor (Nrf2) and heme- oxygenase-1 (HO-1) proteins was examined using specific antibodies on HCT116 cells. Sinigrin (SIN) was the abundant GLs of the six compounds identified and its content together with total aliphatic GLs increased in saline conditions. Fluorescent (FL) and blue plus red (B1R1) lights were identified as stable cultivation conditions for microgreens, promoting biomass and glucobrassicin contents, whereas other identified individual and total indole GLs behaved differently in saline and non-saline environments. Blue light-emitting diodes and FL light in saline treatments mostly enhanced SIN, phenolics and antioxidant activities. The increased SOD and CAT activities render the BC microgreens suitable for lowering oxidative stress. Additionally, activation of Nrf2, and HO-1 protein expression by the GLs rich extracts, demonstrate their potential to treat and prevent oxidative stress and inflammatory disorders. Therefore, effective salt treatments and light exposure to BC microgreens present an opportunity for targeted regulation of growth and accumulation of bioactive metabolites.en_US
dc.identifier.citationMaina, S.; Ryu, D.H.; Cho, J.Y.; Jung, D.S.; Park, J.-E.; Nho, C.W.; Bakari, G.; Misinzo, G.; Jung, J.H.; Yang, S.-H.; et al. Exposure to Salinity and Light Spectra Regulates Glucosinolates, Phenolics, and Antioxidant Capacity of Brassica carinata L. Microgreens. Antioxidants 2021, 10, 1183. https://doi.org/ 10.3390/antiox10081183en_US
dc.identifier.urihttp://www.suaire.sua.ac.tz/handle/123456789/5645
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectBrassicaceaeen_US
dc.subjectBioactive compoundsen_US
dc.subjectReactive oxygen species (ROS)en_US
dc.subjectOxidative stressen_US
dc.subjectAntioxidant enzymesen_US
dc.subjectAntioxidant proteinsen_US
dc.subjectLight wavelengthen_US
dc.titleExposure to salinity and light spectra regulates glucosinolates, phenolics, and antioxidant capacity of brassica carinata L. microgreensen_US
dc.typeArticleen_US
dc.urlhttps://doi.org/10.3390/antiox10081183en_US

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