Evaluation of multiplex pcr for detection of mycobacteria tuberculosis complex microorganisms isolated from cattle tissues and human tuberculosis cases Tanzania

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dc.contributor.authorGabriel, Harrison Sadiki
dc.date.accessioned2026-03-11T12:01:38Z
dc.date.available2026-03-11T12:01:38Z
dc.date.issued2010
dc.descriptionMasters Theses
dc.description.abstractA study on the evaluation of multiplex PCR on detection on Mycobacteria tuberculosis complex was carried on samples obtained from Morogoro district. Specimen collected from abattoir in Morogoro Municipal and human cases from Muhimbili Research Station were analysed at the Faculty of Veterinary Medicine Laboratory by Multiplex PCR. A total of eight nine-pooled lymph nodes samples collected from none tuberculin tested slaughter cattle in the study area-Morogoro abattoir were cultured and Seventy two (80.89%) yielded mycobacteria. Seventeen were identified as M.bovis and three as M.tuberculosis. The remaining fifty two were mycobacteria other than tuberculosis (MOTTs). A total of forty five isolates from human sample were submitted for subculture and thirty eight (84%) were positive for mycobacteria. Twenty five isolated were identified as M.tuberculosis. The remaining thirteen were mycobacteria other than tuberculosis. The IS986 and mtp40 multiplex PCR was able to differentiate M.bovis from M.tuberculosis. The finding of this therefore necessitates the collaboration of veterinary and medical professions to the control transmission of M.bovis infection in human and animal populations.
dc.description.sponsorshipThe Bovine Tuberculosis Project of SUA in collaboration with the Swiss National Science Foundation
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/20.500.14820/7380
dc.language.isoen
dc.publisherSokoine University of Agriculture
dc.subjectMycobacteria tuberculosis
dc.subjectMicroorganisms
dc.subjectCattle tissues
dc.subjectHuman tuberculosis
dc.subjectTanzania
dc.titleEvaluation of multiplex pcr for detection of mycobacteria tuberculosis complex microorganisms isolated from cattle tissues and human tuberculosis cases Tanzania
dc.typeThesis

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